set language = code # pt (Portuguese), es (Spanish), en (English), fr (French), de (German), etc...Commands
Jmol has a multitude of commands for viewing and studying molecular models. Most of these commands are accessed through the Jmol main Menu, or by right-clicking on the program’s main screen or the JSmol applet on the internet. | However, scripting allows the execution of a much larger set of actions via the Console, both of the installed program and of the applet, in addition to allowing the listing of consecutive actions generating specific visualizations, calculations and animations, and even programming. In the latter case, via the Jmol scripting language. These commands and the programming language are explained on the website of its developers, Jmol - Documentation & scripting, with substantial examples and illustrative and interactive links. The content of this chapter aims to summarize the main commands for viewing, studying, importing and exporting, calculations and animations of models via the Console or via script.
As a sequence of commands for a more complex observation or animation, you can create the script in any simple text editor, saving it with the “.spt” attribute. The script can be loaded as a file or pasted into the Console field.
Jmol commands do not distinguish between singular or plural, or between upper or lower case, and both forms can be used, such as show group or Show groups.
An additional note: when creating a script, it is recommended that explanations be provided in command lines; to do so, simply use the hashtag sign (#) after the commands.
As a sequence of commands for a more complex observation or animation, you can create the script in any simple text editor, saving it with the “.spt” attribute. The script can be loaded as a file or pasted into the Console field.
Jmol commands do not distinguish between singular or plural, or between upper or lower case, and both forms can be used, such as show group or Show groups.
An additional note: when creating a script, it is recommended that explanations be provided in command lines; to do so, simply use the hashtag sign (#) after the commands.
Changing the interface language & file directory
Language
The Menu language and the command output (results) can be changed as follows. Remember, however, that the commands themselves refer to computational algorithms; this way, it is not possible to change the language of the commands.
Note: if you are unable to do so, start Jmol by opening a CMD (Windows) or Terminal (Linux) in the program directory folder, and type, for Portuguese:
java -Duser.language=pt -jar Jmol.jarDirectory
cd # shows current directory
cd ? # open window to default directory
cd path # change default directory (e.g. ../temp)
set defaultDirectory "/home/" # default directory (for applet)General commands
Help: help # help zoom
Exit: quit
Undo: undo
Redo: redo
Return to original model (position, size, centering and orientation): reset
Program initialization: initialize
Command history: history on or history off
Minimization by force fields of the model: minimizeTry some of the above commands with the applet for JSmol that follows:
Obtaining molecular models
One model
From the internet:
molecules in general: load $cholesterol
molecules from the PDB (Protein Data Bank): load=9pap
ligands from the RCSB website: load =ligand/hem
compounds from the PubChem website: load ":nafamostat"
From the computer (files must be in the root directory or preselected by "cd" - see above):
load "1aay.pdb"More than one model
Separate: load files "1a2t.pdb" "1aay.pdb"
submit the 1st model: model 1 or model 1.1
present the 2nd. model: model 2 or model 2.1
show all models: model* or frame*
Together: load append "1a2t.pdb" "1aay.pdb"Building simple molecules using SMILES
SMILES, an abbreviation for “Simplified molecular-input line-entry system”, allows you to represent the structure of atomic models using symbols and text (ASCII characters).
Try this formatting at the address below.
https://chemapps.stolaf.edu/jmol/jmol.php?model=CThen replace the model in the link, “model=” with the following SMILES codes, alternatively:
1. Single bond: CC, CN, CO
2. Double bond: C=C, C=N, C=O
3. Triple bond: N%23N, CC%23C
Note: the code for the triple bond is intended to bypass the *SMILES* command, *“#”*, which is interpreted differently in internet addresses.Displaying models:
View: display 2.1
Hide: hide 1.1
Remove models: zap
Shading: set ambient 45 (0-shadow; 100-no shadow)Displaying model information (show command)
Display nothing: show none
Model: show model
Atoms: show atom
Chains (protein or nucleic acid): show chain
Structural minimization information: show DSSP
Surfaces (molecular, electrostatic): show isosurface
Groups: show group
Model information: show info
Protein primary sequence: show sequence
History of commands: show history
Residues (protein): show residues
Molecular orbital: show mo
Internet path: show url
Full PDB file information: show fileMolecular databases
There are several databases for rendering molecular models, and with the possibility of saving the file for use with Jmol.
Notable among these are PubChem or DrugBank for small molecules, and RCSB - Protein DataBank (Research Collaboratory for Structural Bioinformatics) for macromolecules. There is also the IUPHAR/BPS Guide to PHARMACOLOGY, which allows indirect access to both micro and macromolecules. | In any of these, simply look for a field to download 3D molecular models. Jmol accepts around 47 different file types for structural rendering, including PDB, MOL, CIF, SDF, XYZ, SMILES, and AMBER.
General operations
Resetting the model coordinates: reset (size and centering): Shift+ double click (or *reset* in the *Console*)
Removing the Jmol *logo* from images: set frank off
Background: background white
Model resolution: set ambient 10Mouse commands
To open the Menu of options for viewing, calculations and surfaces: right click
Model movements
Simple rotation: click and drag in any direction
Rotation around the same axis up to 360$^{o}$: Shift+right click
Translation of the structure: Ctrl+right click
General magnification (zoom): Shift+left click and drag - zoom in/out - (or mouse, middle button). Motion commands
spin 10 # rotational, with a speed of 10 degrees per frame
spin z -15 # (z axis)
rotate 20 # 20 degrees
rotate x 90 # x axis
translate 50 # value represents the percentage of the window (100 - outside; 0 - center)Saving and exporting models (write)
# (saves as image)
Model image:
default (png): write model-name
with specifications: write PNGJ 2000 2000 "big.png"
others (jpg, pdf): write name.jpg
all models: write frames* "all.jpg"
Model coordinates
write name.mol # or other attribute (e.g. pdb)
Model state (changes after loading): write molecule.spt # of "script"Copying to the clipboard
The model image can be copied through the program’s main Menu.
Reproducing a model from the internet that does not allow saving
Sometimes some sites make molecules available in the *mol or *pdb attribute for viewing and studying, but without the possibility of downloading the file. In these cases, it is possible to obtain the model coordinates, copy them into a notepad, and save the file for reading by Jmol, as *, for example.
1. View the desired model on the site;
2. Right-click and select Console.
3. Click again, select Show and:
Extract mol data.... or
File contentsAs a result, the Console will display the data for copying to a text file. Simply save it as *.mol for future viewing in Jmol.
Quantities
Calculating distances and angles (measure)
In addition to the simple and interactive use of the mouse to calculate distances (double right-click on the 1st atom and drag to the 2nd atom) or angles (3 mouse clicks), it is possible to obtain the same information through the Console.
Distance: measure no. atom1 no. atom2
Angle: measure no. atom1 no. atom2 no. atom 3
Note: to obtain the no. of the atom, simply click on the desired point of the model
Torsion angle: measure atom1 atom2 atom3 atom4
Measurements: measure on..or off...or delete
Choose the unit of measurement: set measure nm # or pm, angstroms, au (Bohr atomic unit)Obtaining & viewing quantities
Hydrogen bonds: calculate hbonds (or...hbonds calculate)
Formal (effective) charges: calculate formalcharge # to label: label %C
Partial charges: calculate partialcharge # to label: label %P
Second structures in a protein: calculate structure # DSSP algorithm - Define Secondary Structure; note: the representation disappears, and it is necessary to type the command again for it to return
All bonds in the model and their respective distances: measure allconnected (*)(*)Changing representations
There are several ways to render structures in Jmol. The commands below summarize this capability:
wireframe, backbone, trace, strands, cartoon, ribbons, meshriboon, rockets, cpk (or spacefill), ball&stick (only through the Menu, or through the combination below in the Console)
To display a single shape: wireframe only
To hide a shape: backbone off
To render as "ball&stick": wireframe only;wireframe reset;spacefill resetThe representations render the structure by the size of its atoms. The rendering by spacefill, for example, is expressed as a function of the van der Waals radius of the atom. For example:
spacefill on / off
wireframe 0.5 # 0.5 Angstrom (the "." refers to the absolute value)
backbone 50 # 50/250 Angstrom, or 0.2 Angstrom (no dot refers to relative value)
cartoon 50% # 50% larger than default
spacefill 2 # 2/250 Angstrom = 0.008 Angstrom (note no dot)
spacefill 2.0 # 2 Angstrom (note dot)Clipping & shading
Clip in view: slab on; slab 30
Depth view: depth 30Whether to display hydrogen or not
Insert into model: set pdbAddHydrogens
Remove from model: set pdbAddHydrogens falseDisplaying disulfide bonds (proteins)
ssbonds on or ssbonds off
ssbonds 40 # renders as rods, with a diameter of 40/250 Angstrom
ssbonds 0.5 # renders with a diameter of 0.2 AngstromJust to practice, try some of the above commands with the applet for JSmol, as follows:
Selecting colors
The default option for model colors is CPK (Corey, Pauling, Koltun), for carbon (gray), nitrogen (blue), phosphorus or sulfur (yellow), hydrogen (white), iodine (violet), etc. However, it is possible to change colors globally in the model, or specifically for selected groups of atoms. The program accepts the command color or colour.
color [255,125,350] # specific color
color bonds # apply the color without needing to specify the representation
color [xFF00FF] # specific color
color cpk # program default
color molecule # all atoms connected by a bond
color formalcharge
color partialcharge
color amino # for amino acids
color structure # for secondary structure
Proteins: red (alpha-helix), yellow (beta-sheets), blue (turns), white (rest)
Nucleic acid: violet (DNA), reddish (RNA)
color chain: chains
color inherit: objects inherit the color of their associated atoms
color shapely: color scheme that includes nucleotides
color group: rainbow gradient (blue; N- or 5'-terminal; red; C- or 3'-terminal)Specific colors
An atom or group of atoms can also be assigned a specific color, as follows:
AliceBlue,AntiqueWhite,Aqua,Aquamarine,Azure,Beige,Bisque,Black,
BlanchedAlmond,Blue,BlueViolet,Brown,BurlyWood,CadetBlue,Chartreuse,
Chocolate,Coral,CornflowerBlue,Cornsilk,Crimson,Cyan,DarkBlue,DarkCyan,
DarkGoldenRod,DarkGray,DarkGrey,DarkGreen,DarkKhaki,DarkMagenta,
DarkOliveGreen,Darkorange,DarkOrchid,DarkRed,DarkSalmon,DarkSeaGreen,
DarkSlateBlue,DarkSlateGray ,DarkSlateGrey,DarkTurquoise,DarkViolet,
DeepPink,DeepSkyBlue,DimGray,DimGrey,DodgerBlue,FireBrick,FloralWhite,
ForestGreen,Fuchsia,Gainsboro,GhostWhite,Gold,GoldenRod,Gray,Grey,Green,
GreenYellow,HoneyDew,HotPink,IndianRed,Indigo,Ivory,Khaki,Lavender,
LavenderBlush,LawnGreen,LemonChiffon,LightBlue,LightCoral,LightCyan,
LightGoldenRodYellow,LightGray,LightGrey,LightG reen,LightPink,LightSalmon,
LightSeaGreen,LightSkyBlue,LightSlateGray,LightSlateGrey,LightSteelBlue,
LightYellow,Lime,LimeGreen,Linen,Magenta,Maroon,MediumAquaMarine,MediumBlue,
MediumOrchid,MediumPurple,MediumSeaGreen,MediumSlateBlue,MediumSpringGreen,
MediumTurquoise,MediumVioletRed,MidnightBlue,MintCream,MistyRose,Moccasin,
NavajoWhite,Navy,OldLace,Olive,OliveDrab ,Orange,OrangeRed,Orchid,PaleGoldenRod,PaleGreen,PaleTurquoise,PaleVioletRed,PapayaWhip,PeachPuff,Turkey,Pink,Plum,
PowderBlue,Purple,Red,RosyBrown,RoyalBlue,SaddleBrown,Salmon,SandyBrown,
SeaGreen,SeaShell,Sienna,Silver,SkyBlue,SlateBlue,SlateGray,SlateGrey,Snow,
SpringGreen,SteelBlue,Tan,Teal,Thistle,Tomato,Turquoise,Violet,Wheat,White,
WhiteSmoke,Yellow,YellowGreen.Labeling the model (label)
Label on/off: label on...or label off
String: label %c
Formal charge: label %C
Partial charge: label %P
Amino acid residue in protein: %n
Residue no.: %r
Atom: %a
Atom identifier no.: %i
Element symbol: %e
String: %cTo view a specific set of atoms, such as residues in a protein, you can use:
select (Ser195,His57,Asp102) and *.ca; label "\%n \%R";color label orange ;set labelfrontSome specific operations also allow labeling.
label binding site # label with the expression
label binding | site # label with the expression, separated by lines ("|" is the "pipe" command)Jmol also allows you to label a specific atom, residue, interaction or reaction site, or some group for better identification and exploration of the model. To do this, you can select the structure by select, followed by the proposed label. For example:
select pro31 # selection of the Proline residue at position 31 of the protein sequence
label Proline-31
set echo top left; echo "Model 1" # insertion of text on the screen
select all; label on # names all atoms with symbol and atomic numberSelecting parts of the model
To select a general group of atoms, simply combine with the mouse Shift+left click+drag for the selected group (you must click on the icon that represents it in the menu first). For a more specific selection, you can use some of the specific or predefined atom expressions, as below:
Nature of the molecule:
all, none, protein, nucleic, dna, rna, carbohydrate, hetero, ligand, solvent, water or hoh, ions, hem, amino
Chemical element
By name: oxygen, nitrogen, carbon
By symbol: _N, _C, _Fe
By atomic no.: elemNo=7, or atomNo=5
By no. atom identification in the sequence (serial): atomNo<50
Part of the molecule:
backbone, sidechain, :b (for chain), spine
Residue type
3-letter abbreviation for amino acids, acidic, basic, polar, neutral, charged, purine, pyrimidine, large, small, buried, surface, cyclic, hydrophilic, hydrophobic, aromatic, cystine, aliphatic, spine, base
Structure 2a. in proteins
helix, sheet, turnThe general commands for using the above expressions or combinations of them are, basically:
select, restrict, hide, display, deleteEach expression must be enclosed in parentheses. You can also apply boolean operations (or, and, but not, or =, <, >, <=, >=, !=).
The restrict command is irreversible in nature, while the hide command is reversible in rendering. followed by the desired expression. Some examples:
Here are some examples:
Restrict command
restrict helix # only keeps the helices of the secondary structure
restrict ligand # only the ligand
restrict not ligand # only the protein without prosthetic groups
restrict 42-60 # only keeps the indicated residue sequence
restrict f6p # only keeps the identified group (a ligand, in this case)
restrict not(:a, :b) # keeps chains other than "a" and "b"
Select command
select Tyr
select polar
select all # selects all atoms
select none # does not select any atoms
select bmp,amp # selects two ligands
select (bmp, amp) and oxygen
select hem and :b # selects the heme group of chain "b"
select hem and not iron :b # selects the heme group of chain "b", omitting Fe
select */2 # all atoms of model 2
select connected(oxygen) # all atoms bonded to an oxygen
select ser70 :A .ca # alpha carbon of serine no. 70 of chain A
select hetero and not solvent # heteroatoms except water
select remove group hetero and not water # only water
select hetero and not ligand # heteroatoms except ligand
select buried only # only protein core residues
select helix or backbone # select helices or protein backbone
select not helix # select other 2nd protein structures (turn, sheet)
select 62 and(sidechain or alpha) # sidechain or alpha carbon of residue 62
select ser70.ca # alpha carbons of serine no. 70
select ser70 :a.ca # alpha carbons of serine no. 70 of chain "a"
select (lys,arg) # only Lys and Arg residues
select (lys,arg) and :b # Lys and Arg residues of chain "b"
select 19-32 :y # sequence of residues of chain "y"
select asp47 # Asp residue at position 47
select [asp]47 :y # asp47 residue of chain "y"
select (10 or 25) and backbone
select resNo>14 and resNo<25 and *.CA # alpha carbons of residues 14 to 25
select hoh.o/2 # oxygen of water molecules in model 2
select (14,16,18,20) # the numbered residues
select backbone and *:b # the backbone of chain "b"
select backbone and not *:b # the backbone of all chains except "b"
select not hidden...select displayed # everything that is not hidden
select not selected # everything that was not previously selected
select carbon and not selected # carbon atoms not previously selected
Delete command
delete(calcium,nadph)Special selections
within command
This command allows a recursive selection, that is, a selection within a selection.
select within (5, (bca,amp)) # selects two ligands that are 5 Angstroms away from the selected group of atoms
select within(3.0, _Fe) # all atoms within 3 Angstroms of the iron atomdefine command
Allows you to define a group of atoms for specific rendering or observation. After defining, simply use select or another command to change the model.
define test (arg10) # select only Arg10
define test (arg10 or asn46) # select both residues
define test (ser195 or asp102 or his57)
define Site within(3.0, _Cu) # 3 Angstroms away from copper atom
define PointX within(5.0,arg31) # 5 Angstroms away from Arg31
define neighbors selected # previously selectedModel magnification (zoom)
There are two basic commands in Jmol for magnification:
- zoom : (expression) size
- zoomTo : time (expression) size
The second command allows an animation of the model, which will be explained in the homonymous section below. Here are some examples for the zoom command.
2x magnification: zoom in
3x magnification: zoom \*3
2x reduction: zoom out
3x reduction: zoom /3
Remove magnification: zoom off
Restrict ligand and magnification: restrict ligand; zoom 0
zoom 100: restores the initial sizeSurfaces
Jmol allows you to render some types of surfaces around the atoms of the model. For macromolecules it should be noted that rendering will take longer.
dots on / off \# van der Waals surface dots only \# only vdw surface present (without model) dots vanderwaals...or dots vdw dots ionic
isosurface on/off # molecular surface
isosurface solvent # excluding solvent
isosurface molecular # including solvent
isosurface molecular 5 # surface at a given VDW radius
isosurface sasurface # surface accessible solvent
isosurface mep # "molecular electrostatic potential"
isosurface map vdw # Van der Waals surface
isosurface map mep # electrostatic potential
isosurface molecular map property PartialCharge # or temperature, vanderwaals
geosurface on/off # molecular surface geodesic (curved) geosurface only # only the surface, without model geosurface vdw
geosurface ionic geosurface 5 # value in Angstrom
Note: To visualize the surfaces including the solvent, you can use the "set solvent on" command first.Graphs
Some two- and three-dimensional graphs can be generated from simple Jmol commands. To return to the original model, issue the model 1 command. Some examples:
plot properties resno partialcharge # resno = residue number (plots after command to calculate surface charge - see above)
plot properties phi psi resno # 3D plot with dihedral angles against residue no. plot rama...or...
plot ramachandran # Ramachandran plot for structure 2a. in proteins
Note: to view the phi and psi angles in the model (not in the plot), type: draw rama (draw off to hide)
Other properties: hydrophobicity, mass, temperature, radius, occupancy, chainoCreating and reading scripts
“Scripting” allows the observation and analysis of a specific part of the model under study, by composing code snippets as command lines. In this way, the script resembles an algorithm with a succession of commands. A script can be loaded by calling program files (File, Open), or even by dragging the file to the program’s screen area.
A Jmol script is based on a Java Script dialect, and can be driven by a sequence of commands on each line of a simple text file, saving it as .spt.
On the other hand, Jmol has its own construction and testing environment, Script Editor, accessible through the main Menu. In this environment, it is possible to create, open, create, check, and run a script.
The Editor can be used when there are several command lines, for better visualization of the algorithmic sequence. In this case, it is also possible to include comments, using:
/* comment */
or
// comment //
or
# commentIn the Programming section, some scripts created in the Editor will be presented.
It is also valid to copy/paste some code snippets in the Console or Jmol/JSmol, separating the commands with semicolons on successive lines. This way, the Script Editor is simulated in the Console itself.
It is also possible through Jmol to define an initial script to be run when the program is opened. To do this:
It is also possible through Jmol to define an initial script to be run when the program is opened. To do this:
set defaultLoadScript "commands"Mutation
It is also possible to observe consequences of mutating specific residues in a model, such as weak interactions (electrostatics or hydrogen bonds, for example).
mutate 33Lys \# specifically changes position 33 of the primary sequence to Lysine mutate Ala34 \~W \# alteral Ala to Trp mutate(1:3) \~GAL \# changes the first three positions in the protein to Gly, Ala and LeuVisualizing regions with strut function
This function allows you to connect atoms in the structure of a protein, showing a dash between them.
select atomno=1392 or atomno=1403
connect
strut
strut 2.0
color strut pinkAnimations
Focusing on the Fe atom in myoglobin: select hem; wireframe only; wireframe 70 \# exclusive rendering of the zooto group 2(\_Fe) 0 \# 2x magnificationZoom in (zoomto)
This feature allows you to view in a magnified and temporally adjusted way mind, some parts of the model of interest, such as ligand interaction sites or prosthetic groups. The syntax of the expression is:
zoomto or zoomTo (atom/group expression) size or...
zoomto time (atom/group expression) sizeExamples:
Zoom in 3x, half a second at a time: zoomto 0.5 *3
Zoom in 4x, half a second at a time: zoomto 0.5 400
Focus on a ligand at 2x magnification: zoomto 2(ligand) 0
Focus on a ligand at 4x magnification, half a second at a time: zoomto 0.5(ligand)* 4Delay
Command that allows you to wait for a certain period (in seconds), before the next action. Normally used in the sequence of commands by the Console, throughout a script.
Move
The move and moveTo commands allow you to combine rotation, translation, and zoom actions on models. The move command reorients the model based on the current coordinates, and has the syntax:
move rotX rotY rotZ zoom dX dy dZ slab timeWhere rot represents the rotation on a given axis, and d the translation on the axis. Examples:
move 90 0 0 0 0 0 0 0 1 - rotates the model 90º on the X axis;
move 0 720 0 0 0 0 0 0 1 - rotates the model two complete turns around the Y axis, during 1 s;
move 90 15 0 100 0 20 0 0 2 - for 2 s, rotates the model by 90º on the X axis and 15º on the Y axis, increases its size to 100, and translates it by 20% of the panel height, all at the same timeMoveTo
Results in an absolute orientation of the model, regardless of its previous coordinates. Its insertion is not simple, since it depends on the orientation data of the model when loaded for the first time, and which can be obtained by:
show orientationAn example of the result of the command is:
moveto /* time, axisAngle */ 1.0 { 616 -708 -346 47.68} /* zoom, translation */ 400.0 0.0 0.0 /* center, rotationRadius */ {15.174467 28.719118 4.726837} 35.148052 /* navigation center, translation, depth */ {0 0 0} 0 0 0 /* cameraDepth, cameraX, cameraY */ 3.0 0.0 0.0;
#OR
#Follows Z-Y-Z convention for Euler angles
reset;center {15.174467 28.719118 4.726837}; rotate z 130.27; rotate y 44.57; rotate z -147.67; zoom 400.0;Note that there are two sets of commands, one for moveTo and the other for reset, center, rotate and translate. To obtain the model in its original coordinates, simply copy one or the other set of data.
Using the first set (moveTo), the line is copied and the animation time is changed, in this case the value 1.0 in “axisAngle */ 1.0”.
Compare
Compares 2 models and reorients the coordinates of the second one to be juxtaposed to the first one, using a correlation algorithm.
load files "$tyrosine" "$epinephrine";
frame *;
compare {2.1} {1.1} rotate translate 5.0Programming in Jmol
Programming and loop commands implemented in Jmol:
IF FOR WHILE STEP BREAK WAIT PAUSE
BREAK CASE CONTINUE QUITLoop (loop)
Allows you to execute a command line repeatedly.
color bonds red; delay 3; color bonds green; loop 1 # the loop is executed every 1 sMolecular modeling
Although quite limited as a tool for molecular modeling purposes, Jmol allows some didactic approaches. The following script exemplifies the tool in modeling. It can be copied and pasted into the Script Editor window, or loaded from a text file.
# Molecular modeling #1
load=3fgu;
define ligbind (ANP, BGC, MG); select ligbind
connect 3.3
strut 0.5
color strut yellow
hide protein or water# Molecular modeling #2
load =3fgu # load structure
cartoon only
hide water
select(BGC,ANP)
wireframe only
select MG
spacefill 100
color palegreen
defines ligbind (ANP,BGC,MG)
select remove hetero and not water
connect 3.3(ligbind and (oxygen or nitrogen)) (selected and (oxygen or nitrogen)) strut yellow # creates lig.H sticks of the protein with the ligands
zoomTo 0.5(ligbind) *4 # zooms every half second, up to 4x at the ligand site
zoom 0; center
navigate 2 DEPTH 30 / ROTATE 120
zoomto (mg) *7
# Note: adapted from Procko, K., Bakheet, S., Beckham, J. T., Franzen, M. A., Jakubowski, H., & Novak, W. R. (2021). Modeling an Enzyme Active Site using Molecular Visualization Freeware. JoVE (Journal of Visualized Experiments), (178), e63170.